Background: Negative attitudes and behaviors toward a person with disability are found among general public and healthcare professionals. Both person disabilities and their caregivers within India are subject to multiple deprivations and limited opportunities in several dimensions of their lives. Aims and objectives: To explore the attitude, behavior, and impact of education in changing attitudes toward persons with disabilities among undergraduate medical students. Materials and methods: A cross-sectional, interventional study was carried out about the effects of education on changing attitude toward persons with disabilities in freshly admitted first-year medical students as a part of the foundation course of Medical Council of India using Multidimensional Attitudes Scale toward Persons with Disabilities (MAS). Results: Female participants have a better attitude toward a person with disabilities than male participants. A negative correlation was observed between age and affect (r = −0.600), cognition (r = −0.552), and behavior (r = −0.689) component scores; also, negative correlation was observed between income and affect (r = −0.175, p = 0.008) and behavior (r = −0.268, p < 0.001) component scores of multidimensional scale. Conclusion: Male participants, younger age, and lower family income of participants were associated with more negative attitudes toward a person with disabilities, and education had a significant impact on developing positive attitude and behavior toward person with disabilities. By training, medical students can bring in a big leap of change in healthcare delivery.